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ATCC
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Image Search Results
Journal: PLoS ONE
Article Title: Structurally simplified biphenyl combretastatin A4 derivatives retain in vitro anti-cancer activity dependent on mitotic arrest
doi: 10.1371/journal.pone.0171806
Figure Lengend Snippet: ( A) Wild-type E6-1 leukemia cells and dominant-negative Fas-associated via death domain (DN FADD) leukemia cells were treated with the indicated drug at the indicated dose for 48 hours and the WST-1 reagent was used to quantify proliferation. Absorbance was read at 450 nm and expressed as a % of DMSO control. (B, C) Cells were treated for either (B) 24 hours or (C) 48 hours and were probed with annexin V and propidium iodide (PI). Percentage of annexin V and PI positive cells was quantified using image-based cytometry. Blue bar represents viable cells; orange bar represents PI and annexin V positive cells; green bar represents annexin V positive cells; red bar represents PI positive cells. Values are expressed as mean ± SD from three independent experiments. * p < 0.05 vs. DMSO control; **** p < 0.0001 vs. DMSO control.
Article Snippet: E6-1 jurkat cells (ATCC, Cat. No. TIB-152, Manassas, VA, USA), an acute T-cell leukemia cell line, as well as a jurkat cell line dominant negative for the
Techniques: Dominant Negative Mutation, Control, Cytometry